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Introduction: Triphenyltetrazolium chloride, also known as 2,3,5-triphenyltetrazolium chloride, tetrazolium red, referred to as TTC, TTZ, or TPTZ, a lipid-soluble light-sensitive complex, can be used for Testing the viability of seeds can also be used to test ischemic infarction in mammalian tissue.

Detection mechanism: The detection mechanism is that TTC itself can act as a redox indicator, and dehydrogenases in living cells (especially succinate dehydrogenase in mitochondria) can reduce TTC. For seeds or plant tissue, the result of staining is that the living tissue is stained with different degrees of red Chemicalbook color, and the dead tissue or inanimate tissue is not stained. For ischemic infarct tissue, it appears pale due to loss of tissue necrosis dehydrogenase activity, while normal tissue appears dark red. The commonly used staining concentration of TTC is 2% (w/v), and the concentration can also be adjusted appropriately according to the tissue type.

Uses: 2,3,5-triphenyltetrazolium chloride is used as a dye in cell biology research.

Uses: Sensitive reagent for reducing sugars; Distinguishing ethanol, ketones and simple aldehydes; Determination of dehydrogenase activity; Titration of diborane, pentaborane and decaborane, etc.; Pesticide residue analysis

Uses: used as analytical reagents and chromatographic analysis reagents