![3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98% Featured Image](https://cdn.globalso.com/xdbiochems/75621-03-3.jpg)
3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
| Catalog Number | XD90075 |
| Product Name | 3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate |
| CAS | 75621-03-3 |
| Molecular Formula | C32H58N2O7S |
| Molecular Weight | 614.88 |
| Storage Details | Ambient |
| Harmonized Tariff Code |
29242970 |
Product Specification
| IR | Comparable to reference |
| Loss on Drying | <2% |
| TLC | One spot |
| Nitrogen | 4.46 - 4.65 |
| Appearance | White powder |
| Solubility (10 % in H2O) | Clear and colourless |
| Conductivity (0.1M in H2O) |
<10us/cm |
| Assay HPLC | >98% |
| UV (5% in H2O) A290nm | <0.5 |
| UV (5% in H2O) A400nm | <0.02 |
| Solvent Content (by GC) Dimethylformamide |
<2.0% |
3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid inner salt use and synthesis method
Biological Activity: CHAPS, a derivative of Cholicacid, is a zwitterionic detergent that solubilizes membrane proteins. CHAPS is used to stabilize various protein-DNA complexes and can retain the biochemical activity of proteins in solution.
Uses: CHAPS is a non-denaturing zwitterionic detergent for membrane biochemistry. Can be used to solubilize membrane proteins and disrupt protein-protein interactions. CHAPS has a low micellar molecular weight (6,150) and a high critical micelle concentration (6-10 mM) and can therefore be removed from the sample by dialysis. It is also suitable for solubilizing proteins in isoelectric focusing and 2D electrophoresis. CHAPS is commonly used in native (urea-free) isoelectric focusing and has been found to give excellent resolution for certain subcellular preparations and plant proteins. Typically concentrations between 2-4% (w/v) are used for isoelectric focusing gels.
Uses: A non-denaturing zwitterionic detergent for membrane biochemistry, which can be used to solubilize membrane proteins and disrupt protein-protein interactions; also suitable for solubilizing proteins in isoelectric focusing and two-dimensional electrophoresis; commonly used in Non-denaturing (no urea) isoelectric focusing; RNA and DNA extraction, no RNase and DNase activity
Uses: Amphoteric surfactant, CHAPS is a non-denaturing zwitterionic detergent, protein lysate for solubilizing membrane proteins and lysing interactions between proteins and proteins.
