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Properties: 3,3',5,5'-Tetramethylbenzidine is white crystalline powder, odorless, tasteless, insoluble in water, easily soluble in acetone, ether, dimethyl sulfoxide, dimethylformamide, etc. Organic solvents.

Preparation: 3,3,5,5-Tetramethylbenzidine is an important chromogen reagent. Using 2,6-dimethylaniline as raw material, through activation, oxidative coupling and purification, pure 3,3,5,5-tetramethylbenzidine is obtained, and the total yield reaches 65%.

Biological activity: TMB (BMblue) is a chromogenic substrate for immunohistochemistry and ELISA.

Uses: This product is a non-carcinogenic substitute for benzidine 1 (Ames test negative), suitable as a peroxidase substrate for enzyme-linked immunosorbent assay. The substrate yields a soluble bluish final product that can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction can be stopped with 2MH2SO4 (turns yellow) and read at 50 nm at 4Chemicalbook. A sensitive and specific reagent for detecting blood, measuring hemoglobin, and measuring peroxidase.

Uses: A new and safe chromogen reagent; TMB has gradually replaced the strong carcinogen benzidine and other carcinogenic benzidine derivatives, and is used in clinical testing, forensic testing, criminal detection and environmental monitoring and other fields; especially in In clinical biochemical tests, TMB, as a new substrate for peroxidase, has been widely used in enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA); it is mainly used in the following projects: occult blood fingerprint detection; Rapid detection of alcohol in saliva; preparation of urine test strips; hepatitis virus detection; pregnancy detection test; rapid determination of glucose, hemoglobin, albumin in blood and urine; fecal occult blood test; determination of granulocyte value in blood, steroids, Detection of sex hormones; determination of enzymatic activity; detection of antigens, antibodies and genetic material