Streptavidin from Streptomyces avidinii CAS:9013-20-1
| Catalog Number | XD90329 |
| Product Name | Streptavidin from Streptomyces avidinii |
| CAS | 9013-20-1 |
| Molecular Formula | C14H17BrClNO2S |
| Molecular Weight | 378.71 |
| Storage Details | -15 to -20 °C |
| Harmonized Tariff Code | 35040090 |
Product Specification
| Appearance | White powder |
| Assay | 99% |
| For research use only, not for human use | research use only, not for human use |
Alkyne-hinged 3-fluorosialyl fluoride (DFSA) containing an alkyne group was shown to be a mechanism-based target-specific irreversible inhibitor of sialidases. The ester-protected analog DFSA (PDFSA) is a membrane-permeable precursor of DFSA designed to be used in living cells, and it was shown to form covalent adducts with virus, bacteria, and human sialidases. The fluorosialyl-enzyme adduct can be ligated with an azide-annexed biotin via click reaction and detected by the streptavidin-specific reporting signals. Liquid chromatography-mass spectrometry/mass spectrometry analysis on the tryptic peptide fragments indicates that the 3-fluorosialyl moiety modifies tyrosine residues of the sialidases. DFSA was used to demonstrate influenza infection and the diagnosis of the viral susceptibility to the anti-influenza drug oseltamivir acid, whereas PDFSA was used for in situ imaging of the changes of sialidase activity in live cells.
