OEM China Da - D-Tryptophan Cas: 153-94-6 99% White or off-white powder – XD BIOCHEM
OEM China Da - D-Tryptophan Cas: 153-94-6 99% White or off-white powder – XD BIOCHEM Detail:
Catalog Number | XD90296 |
Product Name | D-Tryptophan |
CAS |
153-94-6 |
Molecular Formula |
C11H12N2O2 |
Molecular Weight |
204.22518 |
Storage Details | Room temperature |
Harmonized Tariff Code |
29339980 |
Product Specification
Assay | 99% |
Appearance | White or off-white powder |
Spiral tube assembly counter-current chromatography was successfully applied in enantioseparation of dl-tryptophan using bovine serum albumin as chiral selector. An improved biphasic aqueous-aqueous solvent system 12.0% (w/w) polyethyleneglycol 8000-9.0% (w/w) dibasic potassium phosphate-0.1% ammonia-78.9% water was used as the solvent system for counter-current chromatography, in which bovine serum albumin was predominantly distributed in the lower phase of the two-phase aqueous system. The aqueous-aqueous solvent system gave a very high enantioselectivity for d- and l-tryptophan at α=2.605 along with distribution ratio DD=1.200 and DL=0.461. High peak resolution was obtained for enantioseparation of 2.0mg of dl-tryptophan by spiral tube assembly counter-current chromatography under room temperature. It was found that 0.1% ammonia added in the aqueous-aqueous solvent system greatly improved the enantioseparations. An unusual extremely broad peak for l-tryptophan was observed during en antioseparations. In order to give an explanation, mass transfer rates of d- and l-enantiomers through the interface between the two phases were measured. It was found that l-tryptophan showed lower mass transfer rate than d-tryptophan. Further discussions were proposed for possible reasons for mass transfer rate difference between the enantiomers.
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