Hoechst 33258 Cas: 23491-45-4 Pale yellow crystalline powder
| Catalog Number | XD90536 |
| Product Name | Hoechst 33258 |
|
CAS |
23491-45-4 |
|
Molecular Formula |
C25H24N6O·3(HCl) |
|
Molecular Weight |
533.88 |
| Storage Details | -15 to -20 °C |
| Harmonized Tariff Code | 32129000 |
Product Specification
|
Appearance |
Pale yellow crystalline powder |
|
Assay |
99% |
Interaction between antidiabetic drug, Metformin and calf thymus DNA (CT-DNA) in (50mM Tris-HCl) buffer were studied by UV-Visible absorption, fluorescence, CD spectroscopy and viscosity measurements. In fluorimetric studies, the enthalpy and entropy of the reaction between the drug and CT-DNA showed that the reaction is exothermic (ΔH=-35.4522 kJ mol(-1); ΔS=-49.9523 J mol(-1)K(-1)). The competitive binding studies showed that the drug could release Hoechst 33258 completely. The complex showed absorption hyperchromism in its UV-Vis spectrum with DNA. The calculated binding constant, K(b), obtained from UV-Vis absorption studies was 8.3×10(4)M(-1). Moreover, the changes in the CD spectra in the presence of the drug show stabilization of the right-handed B form of CT-DNA. Finally, viscosity measurements revealed that the binding of the complex with CT-DNA could be surface binding, mainly due to groove binding.Copyright © 2012 Elsevier B.V. All rights reserved.
