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Allura red CAS:25956-17-6

Short Description:

Catalog Number: XD90474
CAS: 25956-17-6
Molecular Formula: C18H14N2Na2O8S2
Molecular Weight: 496.422
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Product Detail

Product Tags

Catalog Number XD90474
Product Name Allura red
CAS 25956-17-6
Molecular Formula C18H14N2Na2O8S2
Molecular Weight 496.422
Storage Details 2 to 8 °C
Harmonized Tariff Code 29270000

 

Product Specification

Melting Point 300 °C
Appearance Dark red powder

Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilms produced in vitro by the gram-negative porcine respiratory pathogen Actinobacillus pleuropneumoniae and the gram-positive device-associated pathogen Staphylococcus epidermidis. The effect of PNAG on bulk fluid flow was determined by measuring the rate of fluid convection through biofilms cultured in centrifugal filter devices. The rate of fluid convection was significantly higher in biofilms cultured in the presence of the PNAG-degrading enzyme dispersin B than in biofilms cultured without the enzyme, indicating that PNAG decreases bulk fluid flow. PNAG also blocked transport of the quaternary ammonium compound cetylpyridinium chloride (CPC) through the biofilms. Binding of CPC to biofilms further impeded fluid convection and blocked transport of the azo dye Allura red. Bioactive CPC was efficiently eluted from biofilms by treatment with 1 M sodium chloride. Taken together, these findings suggest that CPC reacts directly with the PNAG matrix and alters its physical and chemical properties. Our results indicate that PNAG plays an important role in controlling the physiological state of biofilms and may contribute to additional biofilm-associated processes such as biocide resistance.


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    Allura red CAS:25956-17-6